DMEM, high glucose, GlutaMAX™ Supplement, pyruvate
This DMEM is modified as follows:
|• High Glucose||• HEPES|
|• Sodium Pyruvate|
|• Phenol Red|
The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle’s Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM product made in our Scotland facility (31966-021). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.
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