This DMEM / F-12 is modified as follows:
|• L-glutamine||• HEPES|
|• Phenol Red||• Sodium Bicarbonate|
The complete formulation is available.Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham’s F-12. This formulation combines DMEM’s high concentrations of glucose, amino acids, and vitamins with F-12’s wide variety of components.
cGMP Manufacturing and Quality System
Gibco™ DMEM / F-12 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ DMEM / F-12 product made in our Grand Island facility (12500-096). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
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