021-66095746
021-66095629
021-66095673

DMEM/F-12, powder

Catalog number: 32500035
Dulbecco’s Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM / F-12 is modified as follows:

With Without
• L-glutamine • HEPES
• Phenol Red • Sodium Bicarbonate

The complete formulation is available.Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham’s F-12. This formulation combines DMEM’s high concentrations of glucose, amino acids, and vitamins with F-12’s wide variety of components.

cGMP Manufacturing and Quality System
Gibco™ DMEM / F-12 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ DMEM / F-12 product made in our Grand Island facility (12500-096). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

لطفاً برای استعلام قیمت

تماس بگیرید