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7500 Real-Time PCR System

Catalog number: 4351105

The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

• Powerful, five-color platform is calibrated for the broadest range of dyes available: FAM™⁄SYBR® Green I, VIC®⁄JOE™, NED™⁄ TAMRA™⁄ Cy3™, ROX™⁄Texas Red™, and Cy5™ dyes
• Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets
• Advanced multi-componenting algorithm minimizes spectral crosstalk – superior for multiplexing
• User-friendly software includes plate set-up wizards, multi-plate data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward

Specifications

Calibrated Dye
SYBR™ Green I, ROX™, NED™, TAMRA™, JOE™, Texas Red™, Cy5™, VIC™, FAM™, Cy3™
Max. Heating Rate (Thermal Block)
2.5°C
Reaction Speed
Standard
Dynamic Range
9 logs
Format
96-well plate
High-throughput Compatibility
Multiplexing
Max. Power
950 W
Optics
CCD Camera, 5 excitation filters, Tungsten-halogen lamp, 5 emission filters
Passive Reference Dye
ROX (Separate Tube), No ROX, ROX (Pre-mixed)
Peak Block Ramp Rate
2.5°C⁄sec
Precision
99.7% confidence (5000 & 10000 copies)
Reaction Volume Range
20-100 µl
Sample Ramp Rate
± 1.1°C⁄sec
Sensitivity
1 copy of RNase P gene (human genomic DNA)
Temperature Accuracy
0.25°C (35°C to 95°C after 3 min)
Temperature Range (Metric)
4-100°C
Temperature Uniformity
0.50°C (after 30 sec)
Thermal Cycling System
Peltier-Based System
Throughput
4 to 5-96-well plates per 8-hr day
Dimensions
Width: 34 cm , Depth: 45 cm , Height: 49 cm

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